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KMID : 0385220010110020041
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Korean Journal of Gerontology
2001 Volume.11 No. 2 p.41 ~ p.48
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Effects of Flavonoids on Amyloid β Peptide Toxicity in PC12 cells
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Kim Seong-Yong
Song Dong-Up
Choi Kee-Oh
Joung Young-Do
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Abstract
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Neurotoxicity of Amyloid β Peptides (Aβ) has been suggested to be important in the pathogenesis of Alzheimer’s disease. Antioxidants such as propylgallate and melatonin have been reported to inhibit the neurotoxicity of Aβ in cultured cells. In this study, effects of tea catechins and some other flavonoids on Aβ toxicity in cultured PC12 cells were examined. Among 13 ftavonoids used, epigallocatechin gallate (EGCG) and epicatechin gallate (ECG) showed the most prominent inhibitory effects with IC5, of 5.6 and 9.4 μM, respectively. The IC50 values for epicatechin (EC) and epigallocatechin (EGC) were 21.2 and 25.2 μM, respectively. The inhibitory potencies of EGCG, ECG, EC, and EGC were greater than those of melatonin and propylgallate. However, quercetin, myricetin, hesperidin, kaempferol, sylimarin, naringenin, luteolin, apigenin, and genistein did not inhibit significantly the A~ toxicity at 0-100 μM. Green tea inhibited potently the Aβ toxicity with IC,, of 0.1 mglml. EGCG, ECG, EC, and EGC were found not to inhibit the Aβ aggregation, as revealed by DNA electrophoresis method. The above results suggest that tea catechins could efficiently inhibit the Aβ neurotoxicity in vivo through their antioxidative activities.
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KEYWORD
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Amyloid β peptide, flavonoids, tea catechins, PC12 cell, cell toxicity
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